“Joint study utilizing DANA to assess the effects of hypoxia on cognitive function. Methods: 21 health, physically fit participants, ages 19-23 years old, were administered a DANA test battery (1) at sea level, (2) immediately after ascending to an elevation over 5,000 meters, and (3) following 16 days of acclimation to such high altitude. The DANA test battery began with a simple reaction time test (SRT1) , followed by a 20-minute series of more complex cognitive tests, and then ended with a second simple reaction time test (SRT2). Two methods were used to quantify the altitude-induced impairment in reaction time: (1) comparing SRT1 scores taken at sea level to SRT1 scores taken immediately after ascending to high elevation (a baseline comparison), and (2) comparing SRT1 scores to SRT2 scores for each DANA testing time point (SRT1 to SRT 2 comparison). Results: No scoring difference was detected between the SRT1 and SRT2 taken at sea level, confirming that DANA testing does not induce sufficient cognitive loading to alter psychomotor performance in healthy participants under normal oxygen levels and barometric pressure. However, there was a significant reduction in scoring between the SRT1 and SRT2 taken immediately after ascension. Further, the SRT1 to SRT 2 comparison from immediately after ascension produced a much larger effect size than the baseline comparison, indicating that such comparison is a robust metric by which cognitive impairment may be quantitatively assessed. Finally, the SRT1 to SRT2 comparison after acclimation to the high altitude resembled that of the sea level SRT1 to SRT2 comparison. Sensitivity and Validity of DANA: DANA is a sensitive measure of changes in cognitive performance due to hypoxia-induced cognitive impairment, and has longitudinal validity to track changes over time. “