“Independent study conducted with participants in the Framingham Heart Study (FHS) and the Boston University Alzheimer’s Disease Research Center (BU ADRC) to establish reference values for, and investigate the association of clinical risk factors with, the digital neuropsychological measures derived from DANA. Methods: 932 FHS participants (average age of 63 years, 57.3% women) formed the reference cohort, and 57 BU ADRC participants (average age of 74, 64.9% women) were included as the validation cohort, with all participants characterized as cognitively intact. All participants completed 3 distinct DANA tests. Results: Sex-specific reference values in 3 age groups (less than 60, 60-69, and 70 and older) of the reference cohort were generated for the digital cognitive measures of each DANA test, and the validation cohort results mostly aligned with those values. Associations between the digital cognitive measures and 19 clinical risk factors were also examined and unique clinical correlations were seen for the different DANA tests. Reference Values Findings: Reference values for participants under age 60 revealed that women exhibited shorter reaction times, indicative for cognitive function, in the DANA test involving verbal memory and attention. However, men displayed quicker reaction times than women in the DANA tests assessing spatial processing and reaction time. In both males and females, reaction times slowed down with increasing age. Clinical Risk Factors Findings: Different digital cognitive measures were significantly associated with varying numbers of clinical risk factors (including cholesterol levels, myocardial infarction and stroke) across the 3 DANA tests, highlighting the need for comprehensive evaluations of clinical risk factors in understanding cognitive performance.”